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Images correction for Auto Absorption

Author: Dr. Anna Amat.

When irradiated, the light emitted by fluorophores can undergo absorption by neighboring particles or self auto-absorption, resulting in a disturbed emission. As a consequence UVl images can result into a wrong interpretation.

This java application (KuMuImgs App) reads the RGB information of each pixel of the VIS, UVR and UVL images and performs a manipulation of the data according to the Kubelka-Munch theory in order to get the corrected response to light.  The corrected image is then displayed and saved.

 

Software requirements: Java and JDK installed (1.5.x and above).